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KMID : 0381120200420121399
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Genes and Genomics
2020 Volume.42 No. 12 p.1399 ~ p.1406
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Correlation between DNA methylation and Thymic Stromal Lymphopoietin expression in asthmatic airway epithelial cells
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Li Yan-Li
Xing Xi-Qian
Xiao Yi
Liu Yan-Hong
Zhou Yu-Shan
Zhuang Min
Li Chao-Qian
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Abstract
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Background: The overexpression of TSLP and DNA methylation in asthma were both risk factors the relationship was not clear.
Objective: This study aimed to investigate the relationship between methylation status of TSLP promoter and mRNA/protein expression in asthmatic airway epithelial cells.
Methods: Human bronchial epithelial cells were cultured in vitro and divided into: Control group, treated with PBS, model group, sensitized with LPS (10 μg/mL) for 12 h (37 °C, 5% CO2). Other groups were cultured with the pCMV3 plasmid (M?+?NC/pCMV), pGPH1 plasmid (M?+?NC/pGPH), DNMT1/pCMV3 plasmid (M?+?DNMT1/pCMV), and DNMT1/pGPH1 plasmid (M?+?DNMT1/pGPH) for 48 h. The expression of DNA methyltransferase 1 and TSLP were measured using real-time PCR and western blotting.
Results: Compared with the control group, TSLP mRNA (1.00?±?0.00 vs. 2.82?±?0.81 vs. 1, P?
Conclusions: Overexpression of TSLP in asthmatic airway epithelial cells may be regulated by DNA demethylation.
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KEYWORD
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DNA methylation, DNA methyltransferase, Thymic stromal lymphopoietin, Bronchial asthma
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